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Monday, October 31, 2005
I tried to change the buffer of my protein by dialysis. And all of my samples were precipitated. Owww... cantiknya. I really don`t have any idea why it could precipitate. I just change phosphate buffer with Tris-HCl buffer having the same pH. And both of them don`t contain any salt at all. I try to find the solution in the internet but I can't find ones.
Tomorrow I want to grow it once more and express the protein. But, instead of using posphate buffer to resuspend the cell pellet, maybe I want to try directly use Tris-HCl buffer. Why lab-work is full with trial and error experiment.... . And life is just another laboratory, isn't it?
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